ThaumatinV1, Main, Exploration, bibRecord, 000106

Purification and characterization of recombinant supersweet protein thaumatin II from tomato fruit.

Identifieur interne : 000106 ( Main/Exploration ); précédent : 000105; suivant : 000107

Purification and characterization of recombinant supersweet protein thaumatin II from tomato fruit.

Auteurs : Aleksey Firsov [Russie] ; Lyubov Shaloiko [Russie] ; Oleg Kozlov [Russie] ; Leonid Vinokurov [Russie] ; Alexander Vainstein [Israël] ; Sergey Dolgov [Russie]

Source :

Protein expression and purification [ 1096-0279 ] ; 2016.

RBID : pubmed:26965414

Descripteurs français

English descriptors

KwdEn :
- Blotting, Western (MeSH), Chromatography, Liquid (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Fruit (chemistry), Fruit (genetics), Lycopersicon esculentum (chemistry), Lycopersicon esculentum (genetics), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (isolation & purification), Plants, Genetically Modified (chemistry), Plants, Genetically Modified (genetics), Recombinant Proteins (chemistry), Recombinant Proteins (genetics), Recombinant Proteins (isolation & purification), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Sweetening Agents (chemistry), Sweetening Agents (isolation & purification), Sweetening Agents (metabolism).
MESH :
- chemical , chemistry : Plant Proteins, Recombinant Proteins, Sweetening Agents.
- chemistry : Fruit, Lycopersicon esculentum, Plants, Genetically Modified.
- genetics : Fruit, Lycopersicon esculentum, Plant Proteins, Plants, Genetically Modified, Recombinant Proteins.
- chemical , isolation & purification : Plant Proteins, Recombinant Proteins, Sweetening Agents.
- chemical , metabolism : Sweetening Agents.
- Blotting, Western, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.

Abstract

Thaumatin, a supersweet protein from the African plant katemfe (Thaumatococcus daniellii Benth.), is a promising zero-calorie sweetener for use in the food and pharmaceutical industries. Due to limited natural sources of thaumatin, its production using transgenic plants is an advantageous alternative. We report a simple protocol for purification of recombinant thaumatin II from transgenic tomato. Thaumatin was extracted from ripe tomato fruit in a low-salt buffer and purified on an SP-Sephacryl column. Recombinant thaumatin yield averaged 50 mg/kg fresh fruit. MALDI-MS analysis showed correct processing of thaumatin in tomato plants. The recombinant thaumatin was indistinguishable from the native protein in a taste test. The purified tomato-derived thaumatin had an intrinsic sweetness with a threshold value in taste tests of around 50 nM. These results demonstrate the potential of an expression system based on transgenic tomato plants for production of recombinant thaumatin for the food and pharmaceutical industries.

DOI: 10.1016/j.pep.2016.03.002
PubMed: 26965414

Affiliations:

Israël, Russie

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Thaumatin, a supersweet protein from the African plant katemfe (Thaumatococcus daniellii Benth.), is a promising zero-calorie sweetener for use in the food and pharmaceutical industries. Due to limited natural sources of thaumatin, its production using transgenic plants is an advantageous alternative. We report a simple protocol for purification of recombinant thaumatin II from transgenic tomato. Thaumatin was extracted from ripe tomato fruit in a low-salt buffer and purified on an SP-Sephacryl column. Recombinant thaumatin yield averaged 50 mg/kg fresh fruit. MALDI-MS analysis showed correct processing of thaumatin in tomato plants. The recombinant thaumatin was indistinguishable from the native protein in a taste test. The purified tomato-derived thaumatin had an intrinsic sweetness with a threshold value in taste tests of around 50 nM. These results demonstrate the potential of an expression system based on transgenic tomato plants for production of recombinant thaumatin for the food and pharmaceutical industries.</div>
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Purification and characterization of recombinant supersweet protein thaumatin II from tomato fruit.

Purification and characterization of recombinant supersweet protein thaumatin II from tomato fruit.

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